Virology & Mycology
Open Access

Fungal-plant interaction for crops improvement under changing environment for food security

International Conference on Mycology & Mushrooms

September 12-14, 2016 San Antonio, USA

Narendra Tuteja

Amity University, India

Keynote: Virol Mycol

Abstract :

For crops improvement under stress conditions in changing environment an understanding of the beneficial fungal-plant interactions is important. Since the stresses lead to reduction in agricultural production, therefore, there is an urgent need to develop stress tolerant crops with no yield loss for future food security. Use of beneficial fungus (root endophyte Piriformospora indica) through non-transgenic and transgenic approaches could be one of the best ways for crop improvement. Through non-transgenic approach P. indica has been found to provide strong growth promoting activity during its symbiosis with a broad spectrum of plants including mustard, pea, tobacco, tomato, rice, lepidium and medicinal plant Coleus forskohlii. Despite its positive impact on the host, little is known about the P. indica genes that may be involved in stress tolerance. However, for transgenic approach to improve the crop under stress condition, first high salinity tolerant genes from P. indica need to be cloned. Recently we have cloned several salinity tolerant genes from P. indica fungus by functional screening, based on random overexpression of a P. indica cDNA library in Escherichia coli grown on medium supplemented with 0.6 M NaCl. Out of these one of the salinity tolerant genes from P. indica (cyclophilin; PiCypA) has been functionally validated for its role in salinity tolerance in bacteria and plant. This gene product catalyzes the inter-conversion of peptidyl prolyl imide bonds in peptide and protein substrates and functions as molecular chaperones. This is also known to be involved in pre-mRNA splicing; however, their RNA binding activity has not been well described. We found that this protein contains unique property of RNA binding. It also provides high salinity tolerance in E. coli. Here we have also shown that the transgenic tobacco plants over expressing fungal PiCypA provide high salinity tolerance and exhibit normal growth under salinity stress conditions. Overall, we demonstrated for the first time a direct evidence of countering salinity stress tolerance in plant by genetic modification using a fungal gene.

Biography :

Narendra Tuteja (PhD, D.Sc., FNA, FNASc., FASc., FNESA, FNAAS, FTWAS), currently working as a Professor & Director, Amity Institute of Microbial Technology, Amity University, Sector 125, Noida, UP - 201313; ntuteja@amity.edu Visiting Scientist and Former Group Leader, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Marg, New Delhi – 110 067, India.

Email: ntuteja@amity.edu