Development of an integrated multimodal instrument for bacterial | 3449
Journal of Physical Chemistry & Biophysics

Journal of Physical Chemistry & Biophysics
Open Access

ISSN: 2161-0398

Development of an integrated multimodal instrument for bacterial colony analysis

International Conference and Trade fair on Laser Technology

July 20-22, 2015 Orlando, Florida, USA

Huisung Kim, Iyll-Joon Doh, Atul K. Singh, Arun K Bhunia, J. Paul Robinson, and Euiwon Bae

Scientific Tracks Abstracts: J Phys Chem Biophys

Abstract :

A forward scattering pattern analysis based bacterial phenol typing, named BARDOT (BActerial Rapid Detection using optical technique), was developed and showed good classification ratio for several bacterial species such as Vibrio, Listeria, Salmonella, and E. coli. Here bacterial colony is considered as an optical modulator, and it renders an incoming plane wavefront to form a forward scattering patterns based on elastic light scattering principle. Since both the macroscopic colony profile and inner optical characteristics of the colony contribute to the pattern, coordinate-matched measurements of the bacterial colony such as morphology, optical density(OD), and the scattering patterns are essential for comprehensive understanding of the bacterial colony. We present a newly designed multimodal BARDOT, which can measure morphology, OD, and scattering patterns of the bacterial colony, utilizing single wavelength (635nm) forward scatterometer with confocal microscope based bacterial colony morphology analyzer. Feasibility test was done for the proposed instrument by comparing the theoretical prediction and experimental forward scattering pattern, and showed promising result.

Biography :

Huisung Kim, received his B.S. from Hongik University in Korea and M.S. from Gwangju Institute of Science and Technology (GIST) in Korea. His current research is on bacteria detection using optical light scattering technique. Before he joins Purdue University in USA, he worked at GIST on development of fiber type confocal microscope module for integrated microscope. His research interest involves laser applications via light scattering for biological samples, mechanical and optical design / control, signal analysis, and instrumentation.