Development of a SYBR Green real-time PCR standard for porcine pa | 9099
Virology & Mycology

Virology & Mycology
Open Access

ISSN: 2161-0517

Development of a SYBR Green real-time PCR standard for porcine parvovirus (PPV) and screening of porcine tissue DNA samples

9th International Virology Congress and Expo

March 13-14, 2017 London, UK

Laxmi Pandey

St. Aloysius College, India

Posters & Accepted Abstracts: Virol Mycol

Abstract :

Swine viral diseases are of great concern as they hamper the economy of the swine industry. One of such major disease problem is porcine parvovirus (PPV) infection and is the major cause of reproductive failures in swine. Porcine parvovirus is an autonomously replicating parvovirus, belongs to genus parvovirus from the Parvoviridae family. Qualitative real time PCR is a method to rapidly and precisely quantify gene activity by detecting RNA level of gene of interest. In the present study we have developed an assay based on real time PCR for the screening of porcine parvovirus in tissue DNA samples. A recombinant plasmid carrying a fragment of VP2 gene was linearized by digesting with Nco I. Serial log 10 dilution of linearized plasmid was prepared and standard curve was generated by using this as the template. A total of 102 DNA samples were screened through real time and conventional PCR, 14 in real time and 4 samples in conventional PCR came positive. Real time PCR found to be 105 times more sensitive than the conventional PCR in PPV negative pig genomic spiking. So we can say that real time PCR though costlier but at the same time it is more sensitive and accurate technique over the conventional PCR.

Biography :