Dental pulp stem cells mobilized with granulocyte colony-stimulat | 52303
Journal of Clinical Trials

Journal of Clinical Trials
Open Access

ISSN: 2167-0870

+44 1478 350008

Dental pulp stem cells mobilized with granulocyte colony-stimulating factor for total pulp regeneration: Phase 1 clinical trial

International Conference on Clinical Trials

July 27-29, 2015 Orlando-FL, USA

Misako Nakashima

ScientificTracks Abstracts-Workshop: J Clin Trials

Abstract :

The dentin-pulp complex does not regenerate spontaneously in pulpectomized adult tooth with complete apical closure as
observed in root canal filling. Our preclinical work has demonstrated therapeutic potential of dental pulp stem cell (DPSC)
subsets isolated by granulocyte-colony stimulating factor (G-CSF)-induced mobilization, known as mobilized DPSCs (MDPSCs)
for complete pulp regeneration. We further assessed the safety and feasibility of autologous transplantation of human MDPSCs in
pulpectomized tooth and evaluated cell therapy in a clinical trial. The trial was approved and monitored by the Japanese Ministry
of Health, Labour and Welfare and by the Ethics Committees. Clinical-grade MDPSCs were isolated and expanded according to
GMP conditions. The MDPSCs with granulocyte-colony stimulating factor (G-CSF) suspended in atelocollagen were transplanted
into pulpectomized tooth. A total of 5 patients with irreversible pulpitis were enrolled and regularly monitored before and after
transplantation for 24 weeks. Excellent quality control of the final individual product, MDPSCs was assured after freezing and
thawing by the lack of contamination, abnormalities/aberrations in karyotype and the expression of stem cell markers, CD29,
CD44, and CD105 and absence of CD31. The clinical laboratory and radiographic evaluations demonstrated no transplant-related
evidence of toxicity and adverse events. Pulp vitality by electronic pulp testing turned a positive response within 4 weeks after
transplantation (n=4). Magnetic resonance imaging (MRI) demonstrated the similar signal intensity in the root canal at week 24
to that in normal pulp. These results suggest that MDPSCs transplantation into the pulpectomized tooth is safe and efficacious for
pulp regeneration in humans.

Biography :

Misako Nakashima has completed her PhD from Kyushu University and Postdoctoral Studies at National Institute of Dental Research, NIH, and Johns Hopkins
University and New York University. She has published more than 80 papers in reputed journals.