Virology & Mycology
Open Access

Dengue virus (DENV) insights using comparative genomics

Joint Event 10^th International Virology Summit & 4^th International Conference on Influenza & Zoonotic Diseases

July 02-04, 2018 | Vienna, Austria

Mansi Verma

Sri Venkateswara College, University of Delhi, New Delhi-110021, India

Posters & Accepted Abstracts: Virol Mycol

Abstract :

DENV is a positive stranded RNA virus composed of three structural proteins which forms the components of virion and seven non-structural proteins which control various functions like viral replication, immunity and pathogenesis. There are five different serotypes of virus namely DENV-1 to DENV-5. So far, no vaccine with higher efficacy has been known for treating all serotypes of dengue. In the present study, comparative genomics of Dengue virus was conducted to explore potential candidates for novel vaccine targets. All ten proteins of all four serotypes of dengue virus were downloaded and analysed for conserved motifs using MEME software. Several B-cell epitopes were predicted using Immune Epitope Database B-cell prediction tool & Bepipred. Overlapping epitopes sequences were analysed for surface accessibility and conservancy. Based on our studies, we could find several conserved epitopes which are antigenic in nature. Using in silico analysis, we could successfully obtain many conserved epitopes common to all serotypes of dengue virus, which seems promising for designing novel vaccine targets.