Transcriptomics: Open Access
Open Access
ISSN: 2329-8936
+44 1223 790975
ISSN: 2329-8936
+44 1223 790975
Zaneta Matuszek, Sylwia A Szczepaniak and Joanna Kufel
Accepted Abstracts: Transcriptomics
Small nucleolar RNAs (snoRNAs) are the best characterized non-coding RNAs transcribed by RNA polymerase II. They are produced as precursors, whose extended 3�?? ends are trimmed exonucleolytically, whereas 5�?? ends either undergo combined endo- and exonucleolytic processing by Rnt1 and Rat1, respectively, or remain unchanged. In the latter case, the m7G cap becomes hypermethylated by Tgs1. cRT-PCR and northern blot analyses suggest that processing of snoRNA 3' and 5' termini is tightly coupled. Inhibition of 5�?? end maturation, in rnt1�? strain, cause the accumulation of snoRNA precursors that don't possess mature 3�?? ends but carry polyadenylated extensions. This defect is further increased when components of the cap-binding complex (CBC) or methyltransferase Tgs1 are missing. Although we observe an accumulation of pre-snoRNAs inrnt1�? strain, there are also mature snoRNAs present. These results suggest the existence of an alternative pathway of 5' end processing that most likely involves presnoRNA cap removal by Dcp1/Dcp2 complex. Interestingly most pre-snoRNAs that accumulates in dcp2�? strain, are not cleaved by Rnt1, which was confirmed by 5´ RACE analysis. Obtained data strongly suggest the existence of a quality control mechanism that coordinates 5�?? and 3�?? end processing. Results were obtained thanks to the financial support given by �??Generacja Przysz�?o�?ci�?� Ministry of Science and Higher Education Funding Programme.
Zaneta Matuszek is a graduate student of the College of Inter-faculty Individual Studies in Science and Mathematics at the University of Warsaw (Poland). She is involved in two majors: biotechnology and chemistry. Since 2011 she has been working in the Institute of Genetics and Biotechnology, University of Warsaw, in a Prof. Kufel�??s lab of RNA metabolism in Eukaryotic cells. She is the organizer of the International Conference Aspects of Neuroscience held annually in Warsaw. Since January 2014 she has been a leader of her own research project granted by �??Generacja Przysz�?o�?ci�?� Polish Ministry of Science and Higher Education Funding Programme.