Journal of Clinical and Experimental Ophthalmology
Open Access

Comparison of effects of activated omental cell with fat derived stem cell on rat limbal corneal alkaline injury

17^th Asia Pacific Ophthalmologists Annual Meeting

September 17-18, 2018 Tokyo, Japan

Athar Shadmani, Masoomeh Eghtedari and Mahboobeh Razmkhah

Shiraz University of Medical Sciences, Iran

Posters & Accepted Abstracts: J Clin Exp Ophthalmol

Abstract :

Purpose: To investigate whether autologous activated omental cells are as effective as fat derived stem cells, in healing process of alkaline burn in limbus of rats. Methods: Corneal alkaline burn was created in three groups of rats (each group was composed of six rats). Then each group were treated differently: Group 1 (control group), with topical medication only, group 2: sub-conjunctivally injected by Autologous Activated Omental Cell (AAOC) after alkaline burn induction , group 3: sub-conjunctivally injected by non-autologous abdominal Fat Derived Stem Cells (FDSC).Clinical outcome was evaluated after 2 months by corneal epithelialization, corneal opacity and neovascularization. Pathologic study was done to access corneal integrity and cell proliferation. Results: After three weeks all three groups had relatively same degree of corneal neovascularization and opacity. But at the time of 2-month follow-up, group 3 rats showed the best clinical results with a more clear healed cornea compared with other groups. All rats on group 1 developed severe central corneal neovascularization and opacity with no healing. Auto evisceration was done in two of them. In group 2: Most cases achieved a clear cornea with peripheral neovascularization two of them developed central corneal neovascularization, but in group 3, none of rats developed central corneal neovascularization and all corneas were relatively clear with no epithelial defects. Conclusion: Activated omental cells are as effective as fat derived stem cells in prevention of corneal neovascularization and wound healing after alkaline burn. Some differences between group 2 and 3 were detected, that should be evaluate in future studies

Biography :

Athar Shadmani is working in Shiraz University of Medical Sciences, Iran

E-mail: drshadmani@gmail.com