Sergey Tarasov, Svetlana Sergeeva1 Oleg Epstein and Jean-Pierre Tafani
Scientific Tracks Abstracts: JAA
The problem of HIV infection treatment is crucial today. Side eff ects of antiretroviral drugs restrict their long-term use, and HIV resistance requires a combined treatment. Searching for new approaches to HIV treatment, APcis (France) conducted an experimental study of anti-HIV activity of compound containing ultralow doses of antibodies (ULDAb) to IFNγ and ULDAb to CD4 (?the drug?), and its combination with AZT (10 nM). Th e study was performed on human mononuclear cells and macrophages inoculated with 100 TCID50/105 cells of HIV- 1-LAI and HIV-1-Ba-L strains, respectively. Anti-HIV activity was assessed based on inhibition of р24 protein concentration and reverse transcriptase (RT) activity. It was shown that the drug administered once 24 hours prior to mononuclear cells inoculation leading to 100% infection or repeatedly during three weeks aft er macrophage inoculation leading to 80% infection, inhibits HIV replication, thus reducing p24 protein concentration and RT activity (vs. control) by 95% and 85% in mononuclears and by 75 % and 78% in macrophages, respectively. Th e combined use of the drug with AZT allow to increase anti-HIV activity of AZT from 67% to 92% in mononuclears aft er 7 days and 82% to 90% in macrophages aft er 17 days while showing no cellular toxicity. Th erefore, the performed in vitro study showed anti-HIV activity of the drug containing ULDAb to IFNγ and ULDAb to CD4, as well as an eventual eff ective use of AZT in lower doses, thus evidencing potentially increased effi cacy and safety of combined antiretroviral therapy due to inclusion of the drug containing ULDAb.
Dr. Sergey Tarasov graduated from Russian Medical University and received MSc degree in biomedical sciences. His master?s dissertation was devoted to the treatment of common pediatric viral infections. He is head of R&D analytical department of Russian pharmaceutical company Materia Medica and specialized in research and development of antiviral agents based on ultralow doses of antibodies. He has published 12 papers listed in MEDLINE database and participated in the International scientifi c conferences such as International Congress of Pediatrics 2007 (Greece), EPHAR 2008 (UK), Europaediatrics 2009 (Russia), ECCMID 2010 (Vienna) etc.