Maivel H Ghattas
Port Said University, Egypt
Posters & Accepted Abstracts: Transcriptomics
Pre-miRNA-499 gene is associated with autoimmune disease miR-449 rs3746444 polymorphisms are inconsistent for Rheumatoid Arthritis (RA). We aimed in this study to investigate association of miR-499 rs3746444 polymorphism with RA activity and severity in Egyptian population. The study of population was conducted as case control study in 100 RA patients diagnosed according to the American College of Rheumatology (ACR) classification criteria for RA, and the control group included 100 healthy subjects who were age-and sex-matched to the RA group. All RA patients were assessed by Disease Activity Score (DAS28). Different genotypes were assessed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). There was significant difference in the allele frequencies between RA patients and control groups. The frequency of minor C allele in RA patients was significantly higher than in the control subjects (P=0.037). C allele carriers were at higher risk of RA than T allele carriers and those who had the heterozygote TC genotype had high C-reactive protein (CRP), disease activity score 28 (DAS 28) and anti- Cyclic Citrullinated Peptide (anti-CCP), while homozygote CC genotype carriers had high Rheumatoid Factor (RF). No significant difference was observed in Erythrocyte Sedimentation Rate (ESR) between different genotypes. Our work suggests that C allele of Pre-miRNA rs3746444 polymorphism contributes to heritability of susceptibility to RA compared to T allele and hence, this polymorphism was associated with the activity and severity of the disease.
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