Centre Hospitalier Universitaire Annaba, Algeria
Posters & Accepted Abstracts: Virol Mycol
Introduction: Fungal infections are a preoccupation becoming more significant in the services in charge of risk patients particularly those invasive. The risk of developing invasive fungal infections depends on the level of patient exposure to sources of fungal spores and their immune status. These infections remain dreadful diseases and their support raises many diagnostic and therapeutic problems. Aim: The purpose of this study is to know the aero fungal flora at high risk services of CHU of Annaba, Medical Intensive Care Unit, large burned Onco-Hematology, Nephrology-Hemodialysis and Internal Medicine. Materials & Methods: 11 surveys were carried out in the services concerned. The applied methods are the sedimentation of airborne spores on agar, swabbing surfaces and the impact on medium in the petri dish with a bio-collector (air sampler). The cultures obtained on Sabouraud medium are identified by filamentation test and Achlamy sporulation test for yeasts and by macroscopic and microscopic examination for filamentous colonies. A transfer into other identification media was realized when necessary (Czapek media, media with extract Malt). Results: Among the isolated yeast-like fungi: The genus Rhodotorula sp., is the predominant type (57%) followed by Candida albicans (27%), of non-albicans Candida species (13%) and Cryptococcus sp. (3%). For filamentous fungi, the genus Aspergillus sp., represents 27% of the isolated strains, followed by the genus Cladosporium sp. (21%), Penicillium sp. (13%), Alternaria sp. and Mucor sp. (11%), Paecylomyces sp. (9%), Fusarium sp. (7%), Scytallidium sp. (5%) and Aureobasidium sp. (3%). Conclusion: Regular surveillance of aerobiocontamination of hospital services allow to assess the impact of environmental risk and evaluate potentially pathogenic fungal species, as well as tracking changes in the species in order to ensure appropriate protection and proper care for invasive fungal infections including invasive aspergillosis.
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