Development and Validation of High-Performance Liquid Chromatography - Diode Array Detector Method for the Determination of Tramadol in Human Saliva

Abdel-Aziz Y. El-Sayed^1*, Khaled M. Mohamed², Maha A.Hilal³, Soheir A.Mohamed³, Khaled E. Aboul-Hagag³ and Ahmed Y. Nasser⁴

¹Chemistry Department, Faculty of Science, Al-Azhar University (Assiut Branch), Egypt, E-mail: mohamedkhaled@gmail.com

²Assiut Chemical Laboratory, Medico-Legal Department, Egypt, E-mail: mohamedkhaled@gmail.com

³Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Sohag University, Egypt, E-mail: mohamedkhaled@gmail.com

⁴Biochemistry Department, Faculty of Medicine, Assiut University, Egypt, E-mail: mohamedkhaled@gmail.com

^*Corresponding Author: Dr. Abdel-Aziz Y. El-Sayed, Chemistry Department, Faculty of Science, Al-Azhar University (Assiut Branch), Egypt Email: