Behaviour of Hydrophobicity of Polymer Based on Ion-Exchange Chromatography
Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

Short Communication - (2022)

Behaviour of Hydrophobicity of Polymer Based on Ion-Exchange Chromatography

Yang Liu*
*Correspondence: Yang Liu, Department of Chemistry, University of Hawassa, Great Rift Valley, Hawassa, Ethiopia, Email:

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About the Study

The hydrophobicity of polymer-based anion converters is a recurring problem in Suppressed Anionic Chromatography (SAC) because it has a negative effect on chromatographic performance. In this, we discuss modified hydrophilic intermediate layers on polyelectrolyte-grafted anion exchangers. A pre-made polyelectrolyte called a Linear Cationic Condensation Polymer (LCCP) was sequentially grafted onto the anion exchangers to functionalize them. The phase capacity and hydrophobicity are both significantly influenced by the carboxylic/hydroxylic intermediate layers that are created during thiol-radical-mediated polymerization. The anion exchangers function well in SAC mode for separating common inorganic anions, polarizable anions, and organic acids [1].

Size-exclusion chromatography and liquid adsorption chromatography, two common methods for separating polymers, cannot offer separations solely depending on the number of deprotonated carboxylic-acid groups and along backbone chain of polymers. The extraction of acid-functional polymers which are soluble in organic solvents is now possible thanks to a revolutionary separation technique built on Non-Aqueous Ion- Exchange Chromatography (NAIEX). The polar, aprotic Nmethyl- 2-pyrrolidone was discovered to be a good solvent [2]. It offers a high relative permittivity (favouring dissociation of ion pairs into free ions) and it is an excellent solvent for polymers and organic salts, such as triethyl-ammonium formate. By deprotonating the carboxylic acid groups in the presence of an organic super base, these polymers acquire a negative charge (tetra methyl guanidine).

Triethylamine and other conventionally strong organic bases lack the base strength necessary to counteract the rise in pKa of polymeric carboxylic acid groups under non-aqueous circumstances. Triethyl-ammonium formate is presented as an alternative to typical salts used only for extraction in hydrophilic ion-exchange chromatography [3]. Strong-anion-exchange columns, which are often used in the industry for separation, were used, and high-temperature evaporative light scattering was used for (almost) universal polymer detection. Based on the polymer's distribution of acid-functionalities, the NAIEX technique produced a separation. For the separation of acidfunctional copolymers, NAIEX was compared to conventional normal- and reversed-phase liquid-chromatography methods.

For such set of 36 anion-exchange columns made by various manufacturers, the principles of the persistence of alkanoic and alkane sulfonic acid homologues were examined. It was investigated how electrostatic and hydrophobic interactions affected the retention and division of organic anions [4]. With a 10 mM sodium hydroxide eluent, the methylene selectivity increases (CH2) for the investigated columns were assessed. On resin hydrophobicity, the effects of the matrix, surface area, polar group structure, ion-exchange capacity, density of charged functional groups on the surface, and other anion-exchanger properties were taken into account.

Whereas, flow-through ion-exchange chromatography is commonly used in bio therapeutic polishing, but the factors leading to impurity persistence are unknown. A large number of dilute impurities with physicochemical diversity may be encountered, making flow-through separation performance super sensitive to process conditions. This study presents two novel correlations that provide transferable additional insight into the chromatographic behaviour of weakly adsorbing impurities. First one, focused on column simulations and experimental validation, distinguishes the relative influence of thermal, transport, and geometric characteristics in determining breakthrough quantities of dilute species. The Graetz amount for mass transfer was discovered to generalize transport contributions, allowing estimation of an equilibrium constant threshold under which impurity perseverance is expected.


This association requires impurity adsorption equilibria, which are often not accessible. The second connection proposed in this paper might be utilized to lessen the experimental load of calculating adsorption equilibrium as a factor of ionic strength. A link among stoichiometric relocation model parameters was discovered by combining isocratic retention data from the literature for over 200 nutrient combinations. This connection, when combined with Yamamoto's analysis of linear gradient elution data, may be used to estimate retentivity roughly from a single experiment results, which might be valuable in estimating host-cell protein chromatographic behaviour.


Author Info

Yang Liu*
Department of Chemistry, University of Hawassa, Great Rift Valley, Hawassa, Ethiopia

Citation: Liu Y (2022) Behaviour of Hydrophobicity of Polymer Based on Ion-Exchange Chromatography. J Chromatogr Sep Tech. S2: 003.

Received: 25-Oct-2022, Manuscript No. JCGST-22-20741; Editor assigned: 28-Oct-2022, Pre QC No. JCGST-22-20741(PQ); Reviewed: 15-Nov-2022, QC No. JCGST-22-20741; Revised: 24-Nov-2022, Manuscript No. JCGST-22-20741(R); Published: 02-Dec-2022 , DOI: 10.35248/2157-7064.22.S2.003

Copyright: © 2022 Liu Y. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.