Journal of Alcoholism & Drug Dependence
Open Access

Laboratory for Morphological and Biomolecular Imaging, Collaborative Research Center, Nippon Medical School, Sendagi, Bunkyo City, Tokyo 113-8602, Japan

Publications

• Research Article   
  Role of Class III Alcohol Dehydrogenase (ADH3) in Renal Pathological Changes Induced by Chronic Alcohol Consumption in Mice
  Author(s): Midori Katsuyama*, Takahisa Okuda, Yoshihiro Sasaki, Masamichi Ishizaki, Kentaro Wada, Motoyo Maruyama, Toshio Akimoto, Youkichi Ohno and Takeshi Haseba
  
  Aims: Our study investigated ADH3 role in renal pathological changes induced by chronic alcohol consumption (CAC). Methods: Nine-week-old malewild-type (WT) and ADH3-deficient (Adh3−/−) mice were administered 10% ethanol solution for 1 month. Renal histological and ultrastructural analyses were performed by light microscopy on periodic acid–Schiff (PAS) staining and electron microscopy. The tissue ADH3 localization was examined by immunohistochemistry using a specific antibody against mouse ADH3. Urine and serum biochemical analyses were performed. Results: The PAS-stained microvilli in the proximal tubules were significantly low in the WT mice after CAC (WT (E)) as compared to the Adh3−/− mice (Adh3−/− (E)). The elect.. View More»
  DOI: 10.35248/2329-6488.21.9.340

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