Journal of Alcoholism & Drug Dependence
Open Access
ISSN: 2329-6488
+44 1223 790975
ISSN: 2329-6488
+44 1223 790975
Department of Legal Medicine, Kanagawa Dental University, Yokosuka, Kanagawa 238-8580, Japan
Research Article
Role of Class III Alcohol Dehydrogenase (ADH3) in Renal Pathological Changes Induced by Chronic Alcohol Consumption in Mice
Author(s): Midori Katsuyama*, Takahisa Okuda, Yoshihiro Sasaki, Masamichi Ishizaki, Kentaro Wada, Motoyo Maruyama, Toshio Akimoto, Youkichi Ohno and Takeshi Haseba
Aims: Our study investigated ADH3 role in renal pathological changes induced by chronic alcohol consumption (CAC).
Methods: Nine-week-old malewild-type (WT) and ADH3-deficient (Adh3−/−) mice were administered 10% ethanol solution for 1 month. Renal histological and ultrastructural analyses were performed by light microscopy on periodic acid–Schiff (PAS) staining and electron microscopy. The tissue ADH3 localization was examined by immunohistochemistry using a specific antibody against mouse ADH3. Urine and serum biochemical analyses were performed.
Results: The PAS-stained microvilli in the proximal tubules were significantly low in the WT mice after CAC (WT (E)) as compared to the Adh3−/− mice (Adh3−/− (E)). The elect.. View More»
DOI:
10.35248/2329-6488.21.9.340