Journal of Bone Research
Open Access
ISSN: 2572-4916
ISSN: 2572-4916
Center for Global Health Research, Saveetha Institute of Medical and Technical Sciences, Saveetha Medical College and Hospitals, Saveetha University, Chennai 600077, India
Research Article
Hypoxic Preconditioning Enhances Mitophagy in BMMSCs
Author(s): Il-Shin Kim* and Ujjal K Bhawal
Background: Bone Marrow-derived Mesenchymal Stem Cells (BMMSCs) have been extensively studied for their therapeutic role in regenerative medicine. However, their effectiveness in BMMSCs-based therapies is constrained due to the hypoxic microenvironment encountered at injured sites. This study aims to investigate the cellular mechanisms that regulate mitochondrial function through mitophagy in hypoxic BMMSCs to improve their therapeutic potential with minimal adverse effects. Material and Methods: C57BL/6 mouse BMMSCs were subjected to a simulated ischemic environment at hypoxia with 1% O2 for 48 hours. Mitophagy markers, including LC3B and BNIP3, were assessed using quantitative real-time PCR, and Western blotting. The hypoxic response was further characterized by evaluating HIF-1α expression and its role in the transcriptional regulation of mitophagy-related genes. Angiogenic f.. View More»