Journal of Applied Pharmacy
Open Access
ISSN: 1920-4159
ISSN: 1920-4159
Department of Basic Medical Sciences, Faculty of Pharmacy, Hajvery University, Lahore, Pakistan
Research Article
A Study to Determine the Effects of Aceclofenac Sodium on Angiogenesis by using Chorioallantoic Membrane (CAM) Assay
Author(s): Anam Nazeer*, Lubna Shakir, Zaka ur Rehman, Owais Omer, Komal Najam, Mohsan Sohail, Nasira Saeed, Tayyaba Nazir, Shawana Aslam and Arshia Batool Khanum
The chick Chorioallantoic Membrane (CAM) is a possibly three-dimensional representation that can be used for in vivo as well as in situ studies. Its relatively easily available, relatively less expensive and consistent in quality render it an appropriate biological model for the use in experiments requiring live tissues. The aim of current research was to determine the angiogenic/antiangiogenic effect of Aceclofenac Sodium (AcS) and the effective dose of AcS. Total 30 fertilized chicken eggs (5 days old) were obtained from a Big bird Pvt. Ltd (local hatchery). Five groups were formed. They were incubated at 37°C with humidity of 55-60%. An opening about 2 cm of diameter was created under aseptic conditions by removing the shell and inner shell membrane. On 6thday of fertilization, Group A was given 0.1 ml Phosphate Buffer Saline (PBS) and served as control, group B, C, D and E wer.. View More»
DOI:
10.35248/2376-0354.20.12.271