Journal of Plant Biochemistry & Physiology
Open Access
ISSN: 2329-9029
ISSN: 2329-9029
National Crops Resources Research Institute (NaCRRI), Namulonge, P.O. Box 7084, Kampala, Uganda
Research Article
In vitro Culture of Heat-treated Anthers Induces Embryogenic Callus in Cassava (Manihot esculenta Crantz)
Author(s): Buttibwa M, Kawuki RS, Oshaba B, Eyokia M, Hershey C, Perera PIP, Heberle-Bors E, Baguma Y and Tugume AK*
Microspore embryogenesis is the developmental plasticity of juvenile male gametophytes to switch from
pollen to embryo development upon exposure to in vitro stress. It is a common method for obtaining
haploid and doubled haploid plants in breeding programs for development of superior varieties via
haploid-diploidization which allows fast development of homozygous lines from heterozygous parents. In
cassava, obtaining haploidization through traditional methods of successive selfing is difficult because of
cassava’s long reproductive cycle, high heterozygosity, and inbreeding depression. As a first step towards
microspore embryogenesis, callus induction following heat treatment has not been investigated in
cassava. We used two elite Ugandan cassava varieties, hereafter called “genotypes”, NASE3 and NASE14,
for the study of callus induction. Heat.. Read More»
DOI:
10.35248/2329-9029.20.8.249