Journal of Physical Chemistry & Biophysics
Open Access
ISSN: 2161-0398
ISSN: 2161-0398
Department of Science, Hamden Hall Country Day School, Hamden, Connecticut, USA
Research Article
Binding Affinities and Cytotoxicity of Novel Psoralen Derivatives: A Fluorescence Spectroscopy Study
Author(s): Cameron M. Puhl and Francis P. Gasparro*
Psoralens are naturally occurring compounds used in clinical settings to treat skin diseases and cancers. Their therapeutic efficacy stems from their ability to intercalate between DNA base pairs, and once activated by ultraviolet A (UVA) light, form mono- and di-adducts that initiate apoptosis. Although a few psoralen derivatives (like 8-MOP, TMP, and AMT) are widely used in treatments such as PUVA (psoralen plus ultraviolet A) and X-PACT (X-Ray Psoralen Activated Cancer Therapy), many other psoralen derivatives with unique structures have not yet been studied. In the present study, we investigated the binding affinities of three novel psoralen derivatives (1B, 6D, and 2F) with a synthetic adenine-thymine polynucleotide (AT-40) using fluorescence spectroscopy. We analyzed the fluorescence quenching data with Scatchard plots to determine the relative binding constants, and then compar.. View More»
DOI:
10.35841/2161-0398.25.15.439