Journal of Drug Metabolism & Toxicology

Journal of Drug Metabolism & Toxicology
Open Access

ISSN: 2157-7609

Bo Feng

School of Pharmacy, Jilin Medical University, Jilin, China

  • Research Article   
    Molecular-Mediated Difference Action of Poloxham124/188: Absorption, Efflux and Suborganelle Accumulation in Caco-2 Cells
    Author(s): Xiaoying Lin, Xinxin Zhou, Feng Hao, Lele Li, Yue Cui, Yanfei Zhang, Jiao Guan*, Heyun Zhu* and Bo Feng*

    Poloxamers (PLs) are commonly used pharmaceutical excipients. According to the different molecular weight, mainly PL124 and PL188 are observed. PL188 with the larger weight, hydrophilic and more application in oral exposure drug delivery systems than PL124. However, the absorption and transport mechanisms of PL188 into intestinal epithelial cells are still unclear. In this study, we study the uptake and efflux of PL124/188 in Caco-2 cells, and subcellular accumulation in Caco-2 cells quantitatived by ultra-high-performance liquid chromatography-triple/time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). The results showed that, the uptake in Caco-2 cells are different: Both PL188 and 124 entered Caco-2 cells through active transport, but PL188 uptake into Caco-2 cells relies on pinocytosis pathway, fosselin-mediated endocytosis pathway, and the endocytosis pathway independent of mestin .. View More»
    DOI: 10.35248/2157-7609.23.14.285

    Abstract HTML PDF

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