Mycobacterial Diseases
Open Access
ISSN: 2161-1068
ISSN: 2161-1068
Department of Biotechnology, The Federal University of Mato Grosso do Sul, Mato Grosso do Sul, Brazil
Short Communication
MALDI-TOF Protocol for M. tuberculosis Identification Improved MALDI-TOF MS Identification of Mycobacterium tuberculosis by Use of a Cell Disruption Protocol
Author(s): Bacanelli GM*, Araujo FR and Verbisck NV
Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) has been used for the identification of Mycobacterium spp. due to its speed, reliability, cost-effectiveness and high efficiency. However, the cell wall of mycobacteria is rich in lipids which makes it difficult to obtain proteins for analysis by MALDI-TOF MS. In this study, two protein extraction protocols were compared: The MycoEx, recommended by the MALDI-TOF instrument manufacturer Bruker Daltonics and the MycoLyser protocol described here in, which used the MagNA Lyser instrument for cellular rupture. Protein extraction with the two protocols was performed using the virulent strain M. tuberculosis H37Rv (NCBI NC_000962) and the results compared. The MycoLyser protocol allowed enhanced Biotyper identification of M. tuberculosis since the scores obtained with this .. View More»
DOI:
10.35248/2161-1068.23.13.359