Mycobacterial Diseases
Open Access
ISSN: 2161-1068
ISSN: 2161-1068
Department of Neuropathology, National Institute of Mental Health and Neuroscience, Bengaluru, Karnataka, India
Research
Exploring Variations in Lipids among Drug-Resistant and Sensitive Mycobacterium tuberculosis by Thin Layer Chromatography and Mass Spectrometry
Author(s): Kavitha Kumar*, Prashant Giribhattanavar, B. K. Chandrasekhar Sagar and Shripad A. Patil*
Mycobacterium tuberculosis (M. tb) lipids are important in the host–pathogen interplay, variation in the lipids organization of cell wall can act as an adaptive response. Specific cell wall structures can possibly result in suboptimal intracellular concentrations of anti-TB drugs, which favors the acquisition of drug resistance. Therefore, lipids from M. tb (drug resistant and sensitive) were analyzed by 2D-thin layer chromatography and mass spectrometry. GraphPad Prism was used to perform Mann-Whitney U test to determine the statistical significance. Difference observed for total lipid content among different resistant isolates was insignificant. However, increase in phospholipids was identified in Multi-Drug Resistant (MDR) isolate compared to sensitive isolate. Isoniazid, streptomycin-isoniazid, and isoniazid-ethambutol resistant isolates showed increased alpha- mycolic acids.. View More»
DOI:
10.35248/2329-9509.24.14.494