Transcriptomics: Open Access
Open Access
ISSN: 2329-8936
ISSN: 2329-8936
ICAR-Directorate of Coldwater department of Fisheries Research, Bhimtal, 263136 Nainital, Uttarakhand, India
Research Article
Differential Gene Expression of Threatened Himalayan Snow Trout Challenged
With Aeromonashydrophilla
Author(s): Ashoktaru*
The snow trout (Schizothorax richardsonii) is endemic to the high altitudinal Himalayan region and thrive well in a wide thermal regime (0-27°C). The species can be used as a model species to study the abiotic and biotic stress response of cold-water fish species. In the present study, we compared the liver transcriptomes of Aeromonas hydrophilla challenged (Ah+) and mock challenged (Ar-) S. richardsonii. The transcriptome database for the species has been developed with a cascade of immune-related differentially expressed genes using RNA sequencing (2×100 bp paired-end) carried out on the Illumina 2000 sequencing platform. Among 50,453 unigenes obtained from de novo assembly, 24,464 unigenes were annotated, among which 82 unigenes were associated with immune responses genes. There were 265 differentially expressed unigenes (189 upregulated, and 75 down-regulated) in Ah+ com.. View More»
DOI:
10.35248/2329-8936.23.9.138