ISSN: 2329-9029
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Department of Plant Biotechnology, Professor of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt
Research Article
Anther Culture in Potato (Solanum tuberosum L.) in vitro
Author(s): AboShama HM and Atwa MM*
Double haploid (DH) plants Production is a valuable tool in plant breeding programs. Since conventional breeding
takes long time for the development of newly improved cultivars, this technique reduces the time needed for this
purpose. Practically, application of (DH) technology in potato breeding through androgenesis in Santana cultivar is
possible by considering number of factors that influence androgenesis. Culture medium has to be considered in
respect of constituents to fulfill the needs of culture target where N6 medium significantly showed better results
(14.61%) if compared to MS medium (8.78%) anthers produced embryos. Auxin/cytokinin represented in this study
by 2,4-D/BA showed better results in induction of embryogenesis when combined together at (2.0 mg/l 2,4-D and
0.5 mg/l BA) which resulted in (35.67%) anthers produced embryos rather than each one of .. View More»