Journal of Molecular Imaging & Dynamics
Open Access

William H. Perman

Publications

• Research Article
  Towards Real-time Metabolic Profiling of Cancer with Hyperpolarized Succinate
  Author(s): Niki M. Zacharias, Christopher R. McCullough, Shawn Wagner, Napapon Sailasuta, Henry R. Chan, Niki M. Zacharias, Christopher R. McCullough, Shawn Wagner, Napapon Sailasuta, Henry R. Chan,
  
  Purpose: The energy-yielding mitochondrial Krebs cycle has been shown in many cancers and other diseases to be inhibited or mutated. In most cells, the Krebs cycle with oxidative phosphorylation generates approximately 90% of the adenosine triphosphate in the cell. We designed and hyperpolarized carbon-13 labeled Succinate (SUC) and its Derivative Diethyl Succinate (DES) to interrogate the Krebs cycle in real-time in cancer animal models. Procedures: Using Parahydrogen Induced Polarization (PHIP), we generated hyperpolarized SUC and DES by hydrogenating their respective fumarate precursors. DES and SUC metabolism was studied in five cancer allograft animal models: breast (4T1), Renal Cell Carcinoma (RENCA), colon (CT26), lymphoma NSO, and lymphoma A20. Results: The extent of hyperpolarization was 8 ± 2%.. View More»
  DOI: 10.4172/2155-9937.1000123

  Abstract PDF

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