Clinical & Experimental Cardiology
Open Access
ISSN: 2155-9880
+44 1300 500008
ISSN: 2155-9880
+44 1300 500008
Ward Y Vanagt
Cardiovascular Research Institute Maastricht CARIM,
Maastricht
England
Research Article
Sildenafil Postconditioning in a Rat Model of Ventricular Fibrillation/ Resuscitation
Author(s): Ward Y Vanagt, Hesam Amin, Arno Brouwers, Chantal C H Pöttgens, Tim G A M Wolfs, Jack P M Cleutjens, Quirine Swennen, Tammo Delhaas and Frits W PrinzenWard Y Vanagt, Hesam Amin, Arno Brouwers, Chantal C H Pöttgens, Tim G A M Wolfs, Jack P M Cleutjens, Quirine Swennen, Tammo Delhaas and Frits W Prinzen
Objective: To evaluate the multi-organ postconditioning potential of sildenafil during resuscitation. Circulatory arrest/resuscitation induces ischemia/reperfusion (I/R) injury in all organs. I/R injury can be reduced using postconditioning during reperfusion. Sildenafil has proven strong single-organ postconditioning properties.Methods: Ventricular fibrillation (VF) was induced and left untreated for 6 min in anesthetized adult male Wistar rats. During resuscitation, placebo (n=10) or intravenous sildenafil 0.2 mg/kg (n=10) was administered. Troponin-i release, lactate release, blood gases and hemodynamic parameters were assessed. After 3 h of reperfusion, rats were euthanized; brain and heart were removed for infarct staining with triphenyltetrazolium chloride. Urinary kidney injury molecule (KIM-1) was assessed. Data expressed as median (inte.. View More»
DOI:
10.4172/2155-9880.1000530