Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
+44 1223 790975
ISSN: 2155-9899
+44 1223 790975
Tigran K. Davtyan
Analytical Laboratory of the Scientific Center for Drug and Medical Technology Expertise,
MH RA, 49/4 Komitas Ave, Yerevan
Armenia
Research Article
The Effect of Antibiotic-Resistant and Sensitive Escherichia coli on theProduction of Pro-Inflammatory Cytokine Response by Human PeripheralBlood Mononuclear Cells
Author(s): Galina V. Volodina, Tigran K. Davtyan, Murat E. Kulmanov, Ardak B. Dzhumagazieva, Sholpan K. Tursunova, Assima O. Abekova, Indira E. Bishimova, Zhansaya S. Abramova, Roza T. Kenzhebekova, Sabina G. Murzageldinova, Ilya S. Korotetskiy and Alexander I. IlinGalina V. Volodina, Tigran K. Davtyan, Murat E. Kulmanov, Ardak B. Dzhumagazieva, Sholpan K. Tursunova, Assima O. Abekova, Indira E. Bishimova, Zhansaya S. Abramova, Roza T. Kenzhebekova, Sabina G. Murzageldinova, Ilya S. Korotetskiy and Alexander I. Ilin
Purpose: A comparative study of the effect of Escherichia coli, differing in gentamicin sensitivity, on the production of human pro-inflammatory cytokines IL-1β, IL-6, TNF-α by human peripheral blood mononuclear cells (PBMC) was carried out to determine the threshold for cytokine response induction. Methods: The multiple drug resistant E. coli strain ATCC-VAA-196 and its derivatives: a gentamicin-resistant E. coli strain R, obtained by prolonged cultivation of bacteria in the presence of antibiotics, and a gentamicin-sensitive strain of E. coli Rev, obtained by prolonged cultivation in the presence of new drug FS-1, were selected for this study. To determine the cytokine minimum inducing concentration (cMIC) for live (carrying DAMPs) and formalin-fixed (exposing canonical PAMPs) E. coli strains, PBMC were co-cultured with different concentrations of bacteria, and the level .. View More»
DOI:
10.4172/2155-9899.1000522