Pharmaceutical Analytical Chemistry: Open Access
Open Access
ISSN: 2471-2698
+44 1478 350008
ISSN: 2471-2698
+44 1478 350008
Sheeba Manoj Nair
Bangalore-560 078, Karnataka
India
Research Article
Development and Validation of High Performance LCMS Methods for
Estimation of Silodosin and Silodosin β-D-Glucuronide in Human Plasma
Author(s): Sheeba Manoj Nair, Ravi Kumar P and Denish KariaSheeba Manoj Nair, Ravi Kumar P and Denish Karia
The aim of present study is to development and validation of analytical methods for estimation of Silodosin and Silodosin β-D-Glucuronidein human plasma in API, method in LCMS. Silodosin is a prescription medication that acts as an alpha-adrenergic blockers used in the treatment for men with benign prostatic hyperplasia, presently commercially available and marketed as Rapaflo in USA and Rapilif, Silodal in other countries worldwide. Drug showed linearity in the concentration range of 0.502 ng/ml to 207.376 ng/ml for Silodosin and 4.121 ng/ml to 302.836 ng/ml for Silodosin β-D-Glucuronide with correlation coefficient consistently greater than 0.99 for Silodosin and Silodosin β-D-Glucuronide. Mass parameters, 496.2/261.0 and 672.2/479.3/261.2 were chosen for analysis by Solid phase extraction method. Different validation parameters to be considered are Accuracy, Precisio.. View More»
DOI:
10.4172/2471-2698.1000118