Andrology-Open Access
Open Access
ISSN: 2167-0250
+44 1300 500008
ISSN: 2167-0250
+44 1300 500008
Rima Dada
All India Institute of Medical Sciences (AIIMS),
New Delhi-110029
India
Research Article
Oxidative Damage to Sperm DNA: Clinical Implications
Author(s): Swetasmita Mishra, Kranthi V, Rajeev Kumar, Neena Malhotra, Kuldeep Mohanty, Vinay Pathak and Rima DadaSwetasmita Mishra, Kranthi V, Rajeev Kumar, Neena Malhotra, Kuldeep Mohanty, Vinay Pathak and Rima Dada
Background: Sperm DNA is susceptible to oxidative damage due to intrinsic and extrinsic factors which cause oxidative stress and due to limited DNA damage detection and repair mechanism. Reactive oxygen species (ROS) are the chief cause of sperm DNA damage. So, this study was planned to assess oxidative stress levels and correlate with sperm DNA damage.
Material and Method: The study included 35 men who had fathered a child in the last one year and 54 male partners of couple experiencing primary infertility. Semen analysis was done according to World Health Organization (1999) criteria. ROS measurement was done by direct chemiluminescence method using luminol as a probe. DNA damage was assessed by sperm chromatin structure assay (SCSA) and expressed as percentage DFI. 8- Hydroxy-2’-deoxyguanosine (8-OHdG) estimation was carried out by competitive ELISA.
Resul.. View More»
DOI:
10.4172/2167-0250.1000116