Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Paulo Renato Zuquim Antas
Laboratório de Imunologia Clínica, Fundação Oswaldo Cruz,
(IOC-FIOCRUZ) RJ
Brazil
Research Article
The Differential In Vitro Presentation of Mycobacterium leprae Antigens by Human Dendritic Cells is determined by the Mechanism of Host Cell Adhesion
Author(s): Dilvani Oliveira Santos, Janaina Guimaraes Coelho, Eloah Christina Lyrio Neri, Ivy de Castro Campos-Souza, Carlos Germano Garrido Ponte and Paulo Renato Zuquim AntasDilvani Oliveira Santos, Janaina Guimaraes Coelho, Eloah Christina Lyrio Neri, Ivy de Castro Campos-Souza, Carlos Germano Garrido Ponte and Paulo Renato Zuquim Antas
Clinical manifestations in leprosy, a chronic infectious disease caused by Mycobacterium leprae, depend on the immunologic reactions of the patient to the bacilli. Previously, we have shown a reversion of the inefficiency of the cellular-mediated immune response against M. leprae in lepromatous leprosy patients when dendritic cells (DCs) were used in vitro as APC. The aim of the current study is to investigate the cell-to-cell interaction when purified human monocytes-derived DCs and macrophages from healthy adult donors were co-cultured with autologous lymphocytes in the presence of either M. leprae or M. bovis BCG by using APCs which were either attached or in suspension. Subsequently, APCs were analyzed by optical microscopy, phenotypically monitored by flow cytometry, and cytokines secretion evaluated by ELISA and Luminex. Overall, our results s.. View More»
DOI:
10.4172/2155-9899.1000443