Clinical & Experimental Cardiology
Open Access
ISSN: 2155-9880
ISSN: 2155-9880
Paula Soler-Ferrer
Spain
Research Article
Utility of Combined Ischemic Pre- and Post-Conditioning to Protect the Human Myocardium
Author(s): Kelly Casós, Paula Soler-Ferrer, Maria Ll Pérez, Juan M Gracia-Baena, Miguel A Castro, Carlos Sureda and Manuel GaliñanesKelly Casós, Paula Soler-Ferrer, Maria Ll Pérez, Juan M Gracia-Baena, Miguel A Castro, Carlos Sureda and Manuel Galiñanes
Objective: Ischemic pre-conditioning (IPreC) and post-conditioning (IPostC) protect the human myocardium against ischemia/reoxygenation (I/R) injury. However, the two interventions may induce variable degrees of protection, suggesting different mechanisms of action. This study assessed whether IPreC and IPostC confer greater protection when used in combination rather than individually.
Methods: The right atrial appendages from 50 patients were subjected to 90 min of ischemia and 120 min of reoxygenation according to different protocols: IPostC (1 cycle of 120 and 180 sec of I/R) and IPreC (1 cycle of 5 min ischemia/5 min reoxygenation), alone and in combination. Lactate dehydrogenase (LDH) release was measured as an index of tissue injury and 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction as an index of cell viab.. View More»
DOI:
10.4172/2155-9880.1000611