Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
+44 1223 790975
ISSN: 2155-9899
+44 1223 790975
Nazzareno Capitanio
Via L. Pinto, c/o OO.RR., 71100 Foggia
Italy
Research Article
Alterations of Mitochondrial Respiration and Complex I Activity in Mononucleate Cells from Psoriatic Patients: Possible Involvement of GRIM-19-STAT3α/β
Author(s): Rosella Scrima, Claudia Piccoli, Giovanni Quarato, Maria Ripoli, Mario Mastrolonardo and Nazzareno CapitanioRosella Scrima, Claudia Piccoli, Giovanni Quarato, Maria Ripoli, Mario Mastrolonardo and Nazzareno Capitanio
Objective: Although the pathogenesis of psoriasis is largely unknown accumulating evidences configure it as an immune-mediated disease determined through cytokines-mediated positive loops between activated lymphocytes subsets and keratinocytes. Mitochondria in addition to their role in the cell bioenergetics are now recognized as a decisional hub in controlling the immunological response. In the present study we compared mitochondria-related functions of PBMC between psoriatic patients and healthy controls.
Methods: Freshly isolated PBMC from eleven psoriatic patients and nine healthy controls were subjected to mitochondria-dependent respiratory activity measurements by high-resolution oxymetry and the specific activity of respiratory chain complexes assessed by spectrophotometric assays. Quantitative RT-PCR and immunoblotting were applied to .. View More»
DOI:
10.4172/2155-9899.1000184