Journal of Glycobiology
Open Access
ISSN: 2168-958X
+44 7868 792050
ISSN: 2168-958X
+44 7868 792050
Marcelo Maraschin
Brazil
Research Article
Pro-angiogenic Activity Assay of Chondroitin Sulfate and Glucosamine Sulfate on Vascular Network of Mouse and of Chick Embryo Chorioallantoic Membrane
Author(s): Fernanda Katharine de Souza Lins Borba, Edbhergue Ventura Lola Costa, Viviane Aparecida Balvedi Polli, Daniela Sousa Coelho, Marcelo Maraschin, Paulo Fernando Dias and Romildo Albuquerque NogueiraFernanda Katharine de Souza Lins Borba, Edbhergue Ventura Lola Costa, Viviane Aparecida Balvedi Polli, Daniela Sousa Coelho, Marcelo Maraschin, Paulo Fernando Dias and Romildo Albuquerque Nogueira
Objective: Target of this study was to test the capacity of chondroitin sulfate (CS) and glucosamine sulfate (GS) to induce in vivo angiogenesis. Methods: The proangiogenic activity of these compounds was analyzed through the assays in chorioallantoic membrane (CAM) of chick embryo and dorsal skin vascularization in mice, but before was realized a cell viability assay with human umbilical veins endothelial cells (HUVEC). Results: In the viability assay, concentrations tested between 30 and 3000 μg/ml showed a reduction of viable HUVEC number. In the CAM assay, CS and GS in an amount 2.0 mg/implant increased the vessels number as compared to control (phosphate buffered saline-PBS). In the assay of the dorsal skin vascularization of adult Swiss mice, the groups treated with CS (2 mg/implant; Gelfoam plug) exhibited an increase in the vessels number into plugs (0.52 ± 0.08 g/dl.. View More»
DOI:
10.4172/2168-958X.1000129