Clinical & Experimental Cardiology
Open Access

Manuel Galiñanes

Publications

• Research Article
  Duration of the Reoxygenation Interval Applied before Ischemic Post-conditioning: Fine-Tuning the Protocol for Human Myocardium
  Author(s): Paula Soler-Ferrer, Kelly Casós, María L Pérez and Manuel GaliñanesPaula Soler-Ferrer, Kelly Casós, María L Pérez and Manuel Galiñanes
  
  Ischemic post-conditioning (IPostC) is a cardioprotection strategy applied after prolonged ischemia. In an in vitro model of human myocardium we previously demonstrated that one cycle of 120 s of ischemia is the most protective; however the optimal duration of the reperfusion interval between prolonged ischemia and the application of IPostC have not been determined. To investigate the importance of this reperfusion interval, myocardial muscles from the right atrial appendage of 26 patients were subjected to 90 min of ischemia followed by 120 min of reoxygenation. IPostC was induced by four different reperfusion intervals (30, 60, 120 and 180 s) followed by 120 s of ischemia. Lactate dehydrogenase leakage and caspase 3 activity were measured to assess cell injury and apoptosis, respectively. The results showed that intervals of 120 and 180 s were more protective than those of 30 and 60.. View More»
  DOI: 10.4172/2155-9880.1000562

  Abstract PDF

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