Journal of Pharmacological Reports
Open Access
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+44-20-4587-4809
Li Tian-De
China
Research Article
Effect of Exogenous Phosphocreatine on Ito, Ina And Ica,L in Ischemic Ventricular Myocytes of Rat
Author(s): Shi Xiang-Min, Li Tian-De, Wang Yu-Tang, Chen Qi, Yang Ting-Shu and Shan Zhao-LiangShi Xiang-Min, Li Tian-De, Wang Yu-Tang, Chen Qi, Yang Ting-Shu and Shan Zhao-Liang
Aim: The aim of this study is to determine the effect of exogenous phosphocreatine (PCr) with different concentration on transient outward potassium (Ito), natrium (INa) and L-type calcium (ICa,L) current in ischemic ventricular cells of rat and to explore its role in the treatment of ischemic heart disease. Methods: Ventricular cells were isolated enzymatically from left ventricular of rat. Peak Ito, INa and ICa,L current were recorded using patch clamp techniques in the whole-cell configuration in the setting of cells superfused with normal Tyrode solution, simple simulated ischemic solution (SI), ischemic solution containing PCr with concentration of 5, 10, 20, 30 mmol/L for 10 min respectively. Results: Compared with simple simulated ischemic solution, peak Ito, INa and ICa,L current and current density of ischemic solution containing PCr of 5, 10, 20, 30 mmol/L significantly imp.. View More»