Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access
ISSN: 2375-4508
+44 1478 350008
ISSN: 2375-4508
+44 1478 350008
Li H
7th Weiwu Road, Reproductive Medical Center, Henan Provincial People's Hospital,
Zhengzhou
China
Research Article
Development of Testes and Expression of β-catenin in Testes Tissue of Mice Born from Vitrified/Cryopreserved Embryos
Author(s): Liang L, Wang L, Hu L, He Q, Zhang C and Li HLiang L, Wang L, Hu L, He Q, Zhang C and Li H
Objective: To evaluate the impact of vitrifying embryo from mice on the development of testes of offspring.
Materials and methods: This study included 60 male offspring mice, 30 male mice from transferred vitrified embryos and 30 male mice from transferred fresh embryos. We tested the morphology and β-catenin gene expression of testes of difference time after they born.
Results: The HE staining results of the testes of the vitrification group and the fresh group showed dark blue staining in the nuclei and varying degrees of red staining in the cytoplasm and fibrous tissue. And there were no significant differences in the morphology of the testis tissues at the different developmental stages between the two groups. With the use of reverse transcription polymerase chain reaction and Western blotting, the data showed.. View More»
DOI:
10.4172/2375-4508.1000197