Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access
ISSN: 2375-4508
+44 1478 350008
ISSN: 2375-4508
+44 1478 350008
Krishna Kalita
Department of A.R.G.O, College of Veterinary Science,
Khanapara, Guwahati-22, Assam
India
Research Article
Effect of different types of in vitro maturation medium (IVM) on maturation rate of non-vitrified and post vitrified-thawed pig follicular oocytes
Author(s): Krishna Kalita, Deka BC, Biswas RK, Barua PM, Borah P, Dutta DJ and Das SKKrishna Kalita, Deka BC, Biswas RK, Barua PM, Borah P, Dutta DJ and Das SK
Purpose: To study the effect of different types of In vitro maturation medium on cumulus cell expansion and nuclear maturation rate of non-vitrified and post vitrified-thawed porcine follicular oocytes.
Methods: Three different In vitro Maturation (IVM) media (IVM-I contained only hormones and growth factors, IVM-II contained porcine follicular fluid along with hormones and growth factors and IVM-III contained porcine follicular fluid, foetal bovine serum and growth factors without any hormone) were prepared using modified tissue culture medium (TCM-199) as a base medium for the study. Porcine oocytes were collected and IVM in above media was done for 48 h.
Results: In case of non-vitrified and vitrified oocytes the rate of cumulus cells expansion did not differ significantly,.. View More»