Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
+44 1223 790975
ISSN: 2155-9899
+44 1223 790975
Jonathan B Angel
Ottawa Hospital-General Campus,
501 Smyth Rd, Room G-12, Ottawa, Ontario, K1H 8L6
Canada
Research Article
An In vitro Model for the Study of HIV Infection of Thymocytes
Author(s): Charlene D. Young and Jonathan B AngelCharlene D. Young and Jonathan B Angel
The study of human thymocytes requires an appropriate matrix to enable the proper function of thymocytes. Although OP9-DL1 cells have been well established as an ideal co-culture system for the generation of T-cells from their progenitors; their ability to support a mixed population of mature human thymocytes for in vitro HIV infection studies has yet to be established. We assessed the effects of co-culturing a heterogeneous population of mature human thymocytes with a mouse derived cell line (OP9) transduced with the notch ligand delta like 1 (OP9-DL-1) and compared this to standard co-culture with human thymic epithelial cells (TEC). Co-culturing thymocytes with OP9- DL1 cells resulted in higher viability and lower apoptosis when compared to TEC co-cultures. The subset distribution and CD127 expression of thymocytes varied slightly between conditions. Thymocytes co-cultured.. View More»
DOI:
10.4172/2155-9899.S7-002