Andrology-Open Access
Open Access
ISSN: 2167-0250
+44 1300 500008
ISSN: 2167-0250
+44 1300 500008
Hueiwang A Jeng
Sc.D. School of Community and Environmental Health,
4608 Hampton Boulevard, Health Sciences Building Room 3140, Norfolk, VA
Tanzania
Research Article
Measurement of 8-Oxo-7,8-Dihydro-2’-Deoxyguanosine in Human Semen
and Urine by Isotope-Dilution Liquid Chromatography-Tandem Mass
Spectrometry with On-Line Solid-Phase Extraction: Comparison with a
Commercial Available Enzyme-Linked Immunosorbent Assay
Author(s): Hueiwang A Jeng, Mu R Chao, Ruei N Li, Chih H Pan and Wen Y LinHueiwang A Jeng, Mu R Chao, Ruei N Li, Chih H Pan and Wen Y Lin
This study aimed to assess the correlation between 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dGuo) in semen and urine, and to compare the analytical methods of the isotope-diluted liquid chromatograph-tandem mass spectrometry (LC-MS/MS) coupled with an on-line Solid-Phase Extraction (SPE) and commercial Enzyme- Linked Immunosorbent Assay (ELISA) used for detecting 8-oxo-dGuo as an oxidative DNA damage marker. Semen and urine samples were simultaneously collected from 85 apparently healthy human subjects. An optimized DNA extraction method was employed to extract DNA from sperm while minimizing oxidation of DNA. All of the biological samples were analyzed by LC-MS/MS and ELISA. All of the biological samples were detected with 8-oxodGuo. ELISA consistently detected two to three times higher 8-oxodGuo levels in urine samples than LC-MS/MS. However, there was no significant correla.. View More»
DOI:
10.4172/2167-0250.1000S1-001