Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Harry Björkbacka
Sweden
Research Article
Apolipoprotein B-100 Peptide p210 Inhibits Proliferation of Naïve T Effector Cells and Promotes Induction of Tolerogenic Antigen Presenting Cells and Regulatory T Cells in Vitro
Author(s): Sara Rattik, Caitriona Grönberg, Maria F Gomez, Harry Björkbacka, Gunilla Nordin Fredrikson, Jan Nilsson and Maria WigrenSara Rattik, Caitriona Grönberg, Maria F Gomez, Harry Björkbacka, Gunilla Nordin Fredrikson, Jan Nilsson and Maria Wigren
Objectives: Modulation of immune responses against LDL antigens through therapeutic vaccines represents a possible new approach for prevention of cardiovascular disease. The mode of action of these vaccines remains to be fully characterized but the protective effect of immunization with the apolipoprotein B-100 (apoB-100) derived peptide p210 has in several studies been associated with activation of regulatory T cells. The present study used an in vitro model to study the effect of p210 on immune cells. Methods and results: CD11c+ antigen presenting cells, CD25-CD4+ naïve T effector cells and CD25+CD4+ T regulatory cells were isolated from mouse spleens using antibody-coated magnetic beads. Pre-incubation of antigen presenting cells with p210 conjugated to cationized bovine serum albumin (p210-cBSA) down-regulated the expression of CD86 and.. View More»
DOI:
10.4172/2155-9899.1000336