Journal of Fundamentals of Renewable Energy and Applications
Open Access

Gregory A. Tucker

Publications

• Research Article
  Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)
  Author(s): Jwan J. Abdullah, Darren Greetham, Nattha Pensupa, Gregory A. Tucker and Chenyu DuJwan J. Abdullah, Darren Greetham, Nattha Pensupa, Gregory A. Tucker and Chenyu Du
  
  This paper explores the possibility of using an industrially processed municipal solid waste (MSW) for cellulase enzyme production via solid state fermentation (SSF) by Trichoderma reesei and Aspergillus niger. Both fungi grew well on the MSW substrate and production of cellulase enzymes was optimized for temperature, moisture content, inoculation and period of incubation. The effect of additional minerals, and alternative carbon and nitrogen sources were also examined. Following optimization a cellulase activity of 26.10 ± 3.09 FPU/g could be produced using T. reesei at 30°C with a moisture content of 60% with an inoculums of 0.5 million spores/g and incubation for 168 hours. Addition of extra nitrogen and/or carbon did not improve cellulase accumulation. Acid or alkali pretreatment of MSW led to reduced cell.. View More»
  DOI: 10.4172/2090-4541.1000206

  Abstract PDF

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