Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access
ISSN: 2375-4508
+44 1478 350008
ISSN: 2375-4508
+44 1478 350008
Elisabetta Tosti
Animal Physiology and Evolution laboratory, Stazione Zoologica Anton Dohrn,
Villa Comunale, 80121 Naples
Italy
Research Article
Sperm DNA Fragmentation Assays Correlate with Sperm Abnormal Morphology and the Pregnancy Outcome
Author(s): Adriana Fortunato, Rita Leo, Sofia Casale, Giuseppina Nacchia, Francesca Liguori and Elisabetta TostiAdriana Fortunato, Rita Leo, Sofia Casale, Giuseppina Nacchia, Francesca Liguori and Elisabetta Tosti
Purpose: A retrospective study of 89 hypo-fertile male patients attending for in vitro fertilization was undertaken in order to evaluate possible correlations among sperm DNA damage, sperm analysis parameters and pregnancies. Methods: Sperm parameters (concentration, normal morphology and multiple anomalies) were evaluated according to the World Health Organization guidelines. DNA damages were simultaneously evaluated on each sperm sample by i) sperm chromatin decondensation test; ii) sperm DNA fragmentation evaluated by Sperm Chromatin Dispersion and Halo sperm Kit; iii) sperm DNA fragmentation evaluated by the Terminal Uridine Nick-End Labelling procedure. Results: Statistical analysis was performed by using analysis of variance and least squares regression. The sperm chromatin dispersion and fragmentation assays showed a statistically significant positive correlat.. View More»
DOI:
10.4172/2375-4508.1000101