Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access

Dwayne A Wise

Publications

• Research Article
  Genomic Instability in Embryonic Stem Cell: A Mechanism for Adaptation and Pluripotency Maintenance
  Author(s): Clara I Esteban-Pérez, Harold H Moreno-Ortiz, Carolina Lucena, Nancy A Reichert and Dwayne A Wise Clara I Esteban-Pérez, Harold H Moreno-Ortiz, Carolina Lucena, Nancy A Reichert and Dwayne A Wise
  
  Embryonic stem (ES) cells have the ability to maintain pluripotency and self-renewal during in vitro maintenance, which is a key to their clinical applications. ES cell quality has been widely evaluated through the determination of their specific genetic and epigenetic profiles. The hypothesis of this study is that genetic stability in repetitive sequences located near key genes involved in pluripotency, self-renewal, differentiation, chromatin assembly, and imprinting could be a signal for adaptation of the ES cell in vitro. Instability in specific repetitive sequences is present and increases during ES cell passages. ES cells displayed significant mean frequencies of instability in twelve markers out of 64 related to pluripotency (OCT4, D1S551), early differentiation (G60405, D18S63, and D1S468), chromatin assembly (D22S447, D6S2252, D10S529, and HISTB2), and imprinting (GRB10-promo.. View More»
  DOI: 10.4172/2375-4508.1000142

  Abstract PDF

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