Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Derek N.J. Hart
Australia
Research Article
Type 1 Diabetic Children and Siblings Share a Decrease in Dendritic Cell and Monocyte Numbers but are Differentiated by Expansion of CD4+T Cells Expressing IL-17
Author(s): Andrew Wilkinson, Lei Bian, Dalia Khalil, Kristen Gibbons, Pooi-Fong Wong, Derek N.J. Hart, Mark Harris, Andrew Cotterill and Slavica VuckovicAndrew Wilkinson, Lei Bian, Dalia Khalil, Kristen Gibbons, Pooi-Fong Wong, Derek N.J. Hart, Mark Harris, Andrew Cotterill and Slavica Vuckovic
Type 1 diabetes (T1D) is an autoimmune disease characterised by multiple defects of immune cells which allow the expansion of pathogenic β cell-specific T effector cells and subsequent T1D development. We performed immunomonitoring assays on blood immune cells in T1D children, their siblings and controls with the supposition that the results would elucidate the sequence of abnormalities in peripheral immune cells leading to the progression of autoimmunity from non-diabetic siblings to diabetic children. We assessed myeloid dendritic cell (MDC), plasmacytoid (P)DC, monocyte, CD4+T and CD8+T cell compartments, and cytokine expression in CD4+T cells and CD8+T cells, by polychromatic flow cytometry in erythrocyte-lysed fresh blood. The numbers of CD16+MDC, PDC and CD16+ monocytes were similarly decreased in diabetic ch.. View More»
DOI:
10.4172/2155-9899.S2-001