Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
+44 1223 790975
ISSN: 2155-9899
+44 1223 790975
Daniela Bau
Germany
Research Article
Solid Phase-Based Cross-Matching Using the Luminex Platform for Solid Organ Allo-Grafting: Rather Regression than Progress in Comparison to the ELISA-Based Precursor Procedure
Author(s): Daniela Bau, Gary Sawers, Anja Wahle, Wolfgang Altermann and Gerald SchlafDaniela Bau, Gary Sawers, Anja Wahle, Wolfgang Altermann and Gerald Schlaf
Antibodies directed against HLA antigens of a given donor represent the most prominent cause for hyper-acute and acute rejections. In order to select recipients without donor-specific antibodies the complement-dependent cytotoxicity (CDC-) crossmatch was first established representing the standard procedure up to the present. Its negative pre-transplant outcome is currently regarded as the most important requirement for a successful short term kidney graft survival. As a functional assay, however, it strongly depends on the availability of isolated donor lymphocytes and in particular on their vitality. Moreover, during the last ten years several disadvantages of the CDCbased procedure have increasingly been discussed with respect to this assay’s high susceptibility to disruptive factors which frequently lead to false positive outcomes. In this context several autoimmune diseases.. View More»