Emergency Medicine: Open Access
Open Access
ISSN: 2165-7548
ISSN: 2165-7548
Chin Hsu
People's Republic of China
Research Article
Transcriptional Regulation of the Group IIA Secretory Phospholipase A2 Gene by C/EBPδ in Rat liver and its Relationship to Hepatic luconeogenesis during Sepsis
Author(s): Rei-Cheng Yang, Chin Hsu, Tzu-Ying Lee, Kung-Kai Kuo, Shou-Mei Wu, Yen-Hsu Chen, Mei-Ling Ho, Xing-Hai Yao, Chia-Hsiung Liu and Maw-Shung LiuRei-Cheng Yang, Chin Hsu, Tzu-Ying Lee, Kung-Kai Kuo, Shou-Mei Wu, Yen-Hsu Chen, Mei-Ling Ho, Xing-Hai Yao, Chia-Hsiung Liu and Maw-Shung Liu
Background
The present study was undertaken to test hypothesis that altered transcription of secretory Phospholipase A2 (sPLA2) gene in rat liver is regulated by CCAAT/enhancer binding protein δ (C/EBPδ), and to assess its relationship to hepatic gluconeogenesis during the progression of sepsis.
Methods
Sepsis was induced by Cecal Ligation and Puncture (CLP). Experiments were divided into three groups, control, early sepsis (9 h after CLP), and late sepsis (18 h after CLP).
Results
DNA mobility and super shift assays reveal that C/EBP complexes in the liver consisted of at least three isoforms: C/EBPα, C/EBPβ, and C/EBPδ; and various C/EBP isoforms were capable of interacting with each other. Hepatocyte transfection experiments demonstrate that under normal condition.. View More»
DOI:
10.4172/2165-7548.1000151