Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
+44 1223 790975
ISSN: 2155-9899
+44 1223 790975
Basil M Hantash
Escape Therapeutics, Inc.,,
5941 Optical Court, San Jose, CA 95138
Tanzania
Research Article
Novel Negative Selection Marker CD54 Enhances Differentiation of Human Adipose-Derived Mesenchymal Stem Cells
Author(s): Longmei Zhao, Poria Abdollah, Sylvia Do, Chris Nye and Basil M HantashLongmei Zhao, Poria Abdollah, Sylvia Do, Chris Nye and Basil M Hantash
Due to their multi-differentiation potential and immunosuppressive function, mesenchymal stem cells (MSCs) hold huge promise in regenerative medicine. Lack of specific selection markers to isolate MSCs renders their use at risk of fibroblast contamination. The aim of the study was to identify new surface protein markers that can be used for MSC purification during in vitro expansion. With real-time RT-PCR, we demonstrated that primary human dermal fibroblasts expressed CD54 mRNA 10-fold more than early passage human adipose-derived MSCs (AMSCs). Flow cytometry illustrated 88.0% ± 4.1% of dermal fibroblasts strongly expressed CD54 on their surface with a mean fluorescence intensity ratio of 24.0 ± 0.0 compared to 11.0% ± 0.7% and minimal intensity for AMSCs. Evaluation of CD54 sorted AMSCs revealed CD73 expression was 2.2-fold higher in the CD54- versus CD54+ fraction. C.. View More»
DOI:
10.4172/2155-9899.S9-005