Cloning & Transgenesis
Open Access
ISSN: 2168-9849
ISSN: 2168-9849
Ajay BC
Centre for Viticulture and Small Fruit Research,
6505 Mahan Drive Tallahassee, Florida 32317
Tanzania
Research Article
Isolation and Characterization of Inositol Tetraphosphate 1-Kinase (AhITPK1) and Inositol 1,4,5-Tris-Phosphate Kinase (AhIPK2) Gene in Peanut
Author(s): Ajay BC, Ananga A, Kambiranda D, Obuya J, Bera SK and Tsolova VAjay BC, Ananga A, Kambiranda D, Obuya J, Bera SK and Tsolova V
In the current study, partial cDNA clones of inositol tetraphosphate 1-kinase (ITPK1) and inositol 1,4,5-trisphosphate kinase/inositol polyphosphate multikinase (IPK2), were isolated from embryo using RT-PCR and designated as AhITPK1 and AhIPK2 isoforms of the gene. The partial cDNA sequence of AhITPK1 and AhIPK2 genes have an open reading frame (ORF) of 1146 and 891bp respectively and showed high similarity to other plant genes. AhITPK1 shared high homology with Aradu. Q95MC of Arachis duranensis, had a single exon with no introns and belonged to ATP-grasp family of proteins. AhIPK2 shared high similarity with Aradu.24V9G of A. duranensis and contained three exons with 5’ and 3’ UTR’s on either side. Unlike other IPK2 genes, AhIPK2 possessed conserved domains such as PxxxDxKxG and [L/M][I/V]D[F/L][A/G][H/K]. Phylogenetic analysis grouped AhITPK1 with A. duranensis, .. View More»
DOI:
10.4172/2168-9849.1000150