Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
+44 1223 790975
ISSN: 2155-9899
+44 1223 790975
Aimbire F
UNIFESP Institute of Science and Technology,
Rua Talim, no. 330-Vila Nair, CEP: 12231-280. São José dos Campos SP
Brazil
Research Article
The M1/M2 Pattern and the Oxidative Stress are Modulated by Low- Level Laser in Human Macrophage
Author(s): Carvalho JL, Britto A, Souza NH, Ligeiro de Oliveira AP, Anatriello E, Albertini R and Aimbire FCarvalho JL, Britto A, Souza NH, Ligeiro de Oliveira AP, Anatriello E, Albertini R and Aimbire F
Objective: This study investigated the phototherapy (PhT) effect on U937 cells activated by lipopolysaccharide (LPS) from E. coli. PhT has been reported to control the release of inflammatory mediators from different cells activated by LPS. It is unknown if the macrophage M1/M2 pattern as well as the reactive oxygen species (ROS) generation and the pro- and anti-inflammatory cytokines secretion from U937 cells can be influenced by PhT.
Methods: The U937 cells, a human monocytic cell line, were cultured and matured to macrophages in a medium with LPS and irradiated (660 nm) at 4.5 J/cm2. Apoptosis was standardized with Annexin-V and propidie iodate (PI) and the cytotoxicity assay evaluated by MTT. ROS was measured by DCFH-DA. The cytokines, chemokines, NF-κB and Sp1 activity were measured by ELISA. PhT was studied in the presence of a S.. View More»
DOI:
10.4172/2155-9899.1000387