Journal of Chromatography & Separation Techniques
Open Access

Abstract

To Identify the Concentration Level of Various Pigments & to Determine Suitable Solvent System for Different Lipstick Samples by Using TLC

Sonal Srivastava, Kapil Verma and Jyoti Singh

30 lipstick samples of different brands of similar color were selected for this study coloring agent was analyzed by thin layer chromatography (TLC) and UV. Using four different solvent systems [Toluene/Benzene (12:8), Toluene/ Acetone (16:4), Toluene/Benzene/Cyclohexane (4:12:4), Toluene/Benzene/Diethyl ether (12:6:2)]. Lipstick samples of colors indistinguishable on visual observation could be grouped into eight subgroups. The work that follows goes on this line of investigation. The study compares the usefulness of two kinds of fluorescent reagents -Yellowescent Fluorescent Latent Prints Powder and Nile Red- for developing latent lip prints, older than one and a half year, on multi colored surfaces. The reagents were used in powder form and luminescence was observed by an alternate light source and ultraviolet light. In principle Nile Red, like lysochromes, have advantage over other Fluorescent powders because it react with fats and physical agents. The main purpose of this study is to identify concentration level of various pigments & to determine which solvent system is best for the particular brand of the lipstick using Thin Layer Chromatography by checking the differences in the respective chromatogram & UV.