Gui-Ying Meia,b, Joshua Tanga , Christine M. Careya , Susan Tosha , Magdalena Kostrzynska
Probiotics and prebiotics are known to benefit the host through modulating immune functions in multiple ways. In this study, selected probiotic lactic acid bacteria strains, various prebiotics, and synbiotic combinations of probiotics and prebiotics were investigated for anti-inflammatory effects in in vitro models. Secreted interleukin-8 (IL-8) from HT-29 cells was monitored as an inflammation parameter. Individually, the prebiotics raffinose, stachyose, verbascose, oligomate 55NP and beta-glucan decreased the secretion of IL-8 from stimulated HT-29 cells, as compared to stimulated HT-29 medium controls. With the exception of Bifidobacterium longum ATCC 15703, all the tested probiotic bacteria individually reduced secreted IL-8 levels. Synbiotic combinations of the tested probiotics with raffinose, soy extract or oligomate 55NP significantly reduced the IL-8 production in stimulated HT-29 cells, as compared probiotics alone. Several symbiotic combinations of stachyose, verbascose, fructooligosaccharides (FOS), inulin or beta-glucan with selected probiotic strains, decreased the secretion of IL-8. The combination of B. breve (FRP 334) with the GOS- type prebiotics, raffinose, stachyose, verbascose, oligomate 55NP, soy extract or galactobiose all significantly decreased the secretion of IL-8. Thus, this study demonstrated the ability of raffinose family oligosaccharides (raffinose, stachyose verbascose alone or in combination with probiotic bacteria to exert antiinflammatory effects and thus modulate the immune system. In addition, the mixture of several oligosaccharides, such as soy extract, shows great ability to modulate immune function when combined with probiotics compared to the synbiotics of pure oligosaccharides with probiotics.
Published Date: 2021-09-06;